MICROBIOLOGY - THEORY
1. Introduction to the science of microbiology. Major divisions of microbial world and Relationship among them.
2. Different methods of classification of microbes & study of Bacteria , Fungi, virus, Rickettsiae, Spirochetes.
3. Nutritional requirements, growth and cultivation of bacteria and virus. Study of different important media required for the growth of aerobic and anaerobic bacteria & fungi. Differential media, enriched media and selective media, maintenance of lab cultures
4. Different methods used in isolation and identification of bacteria with emphasis to different staining techniques and biochemical reactions. Counting of bacteria Total and Vi able counting techniques
5.Detailed study of different methods of sterilization including their merits and demerits. Sterilization methods for all pharmaceutical products. Detailed study of sterility testing of different pharmaceutical preparations . Brief information on Validation.
6. Disinfectants: Study of disinfectants, antiseptics, fungicidal and virucidal agents factors affecting their activation and mechanism of action. Evaluation of bactericidal, bacteristatic, , virucidal activities, evalua preservatives in pharmaceutical preparations
7. Immunology: Immunity, Definition, Classification, General principles of natural immunity, Phagocytosis, acquired immunity( active and passive ) . Antigens, chemical nature of antigens structure and formation of Antibodies, AntigenAntibody reactions. Bacterial exotoxins and endotoxins. Significance of toxoids in active immunity, Immunization programme, and importance of booster dose .
8. Diagnostic tests:
9. Microbial culture sensitivity Testing: Interpretation of results Principles and methods of different microbiological assays, microbiological assay of Penicillin, S treptomycin vaccines and sera.
10. Study of infectious diseases:
PRACTICAL SYLLABUS
1. Study of apparatus used in experimental microbiology*.
2. Sterilisation of glassware, preparation of media & Sterilisation
3 Staining techniques
4 Study of motility characters*.
5 Enumeration of micro-organisms (Total and Viable)*
6 Study of the methods of isolation of pure culture.*
7 Bio chemical testing for the identification of micro*-organisms
8.Cultural sensitivity testing for some micro-organisms.*
9 Sterility testing for powders and liquids.*
10 Determination of minimum inhibitory concentration.*
11 Microbiological assay of antibiotics by cup plate method.*
12 Microbiological assay of vitamins by Turbidometric method**
13 Determination of RWC.**
14 Diagnostic tests for some common diseases, Widal, malarial parasite.**
* Indicate minor experiment
** indicate major experiment
1. Introduction to the science of microbiology. Major divisions of microbial world and Relationship among them.
2. Different methods of classification of microbes & study of Bacteria , Fungi, virus, Rickettsiae, Spirochetes.
3. Nutritional requirements, growth and cultivation of bacteria and virus. Study of different important media required for the growth of aerobic and anaerobic bacteria & fungi. Differential media, enriched media and selective media, maintenance of lab cultures
4. Different methods used in isolation and identification of bacteria with emphasis to different staining techniques and biochemical reactions. Counting of bacteria Total and Vi able counting techniques
5.Detailed study of different methods of sterilization including their merits and demerits. Sterilization methods for all pharmaceutical products. Detailed study of sterility testing of different pharmaceutical preparations . Brief information on Validation.
6. Disinfectants: Study of disinfectants, antiseptics, fungicidal and virucidal agents factors affecting their activation and mechanism of action. Evaluation of bactericidal, bacteristatic, , virucidal activities, evalua preservatives in pharmaceutical preparations
7. Immunology: Immunity, Definition, Classification, General principles of natural immunity, Phagocytosis, acquired immunity( active and passive ) . Antigens, chemical nature of antigens structure and formation of Antibodies, AntigenAntibody reactions. Bacterial exotoxins and endotoxins. Significance of toxoids in active immunity, Immunization programme, and importance of booster dose .
8. Diagnostic tests:
- Schick's test,
- ELISA TEST,
- Western blot test,
- Southern Blot test,
- PCR ,
- Widal test,
- Mantaux peripheral smear,
- Study of Malarial parasite.
9. Microbial culture sensitivity Testing: Interpretation of results Principles and methods of different microbiological assays, microbiological assay of Penicillin, S treptomycin vaccines and sera.
10. Study of infectious diseases:
- Typhoid,
- Tuberculosis,
- Malaria,
- Cholera,
- Hepatitis,
- Meningitis,
- Syphilis & Gonorrhea
- HIV
PRACTICAL SYLLABUS
1. Study of apparatus used in experimental microbiology*.
2. Sterilisation of glassware, preparation of media & Sterilisation
3 Staining techniques
4 Study of motility characters*.
5 Enumeration of micro-organisms (Total and Viable)*
6 Study of the methods of isolation of pure culture.*
7 Bio chemical testing for the identification of micro*-organisms
8.Cultural sensitivity testing for some micro-organisms.*
9 Sterility testing for powders and liquids.*
10 Determination of minimum inhibitory concentration.*
11 Microbiological assay of antibiotics by cup plate method.*
12 Microbiological assay of vitamins by Turbidometric method**
13 Determination of RWC.**
14 Diagnostic tests for some common diseases, Widal, malarial parasite.**
* Indicate minor experiment
** indicate major experiment
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